Human lifetime CD34+ cell scRNA-seq

In this joint effort with Grant Rowe’s lab, we perform single cell RNA-seq (InDrop platform) on 58,041 CD34+ human hematopoietic stem and progenitor cells (HSPCs) spanning first trimester fetal hematopoiesis through elderly hematopoiesis. The following interactive web interface allows users to browse and compare HSPC gene expression data throughout human life:

Link to the data

(https://lifetimecd34.hlilab.org)

Paper citation:

The dynamics of hematopoiesis over the human lifespan

Li H*, Côté PD, Kuoch M, Ezike J, Frenis K, Afanassiev A, Greenstreet L, Tanaka-Yano M, Tarantino G, Zhang S, Whangbo J, Butty VL, Moiso E, Falchetti M, Lu K, Connelly GG, Morris V, Wang D, Chen AF, Bianchi G, Daley GQ, Garg S, Liu D, Chou ST, Regev A, Lummertz da Rocha E, Schiebinger G, Rowe RG*. Nature Methods. 2024. *co-corresponding